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The dosage for each drug across all groups was standardized at 5 mg/kg to ensure consistency in the assessment

Slides were mounted with neutral balsam (Sinopharm Chemical Reagent Co, Ltd.)

10.1038/nature07813 15 ChenB.LiJ.ZhuH

In this model system, when we performed cytotoxicity assays in the presence or absence of 10 M valspodar, a P-gp inhibitor, we observed that P-gp overexpression conferred resistance to imidazole ketone erastin, piperazine erastin, and FIN56, and that valspodar reversed the resistance

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2 Further research has also demonstrated that GST is expressed abundantly in tumor cells and associated closely to carcinogenesis, tumor formation, and chemotherapy resistance