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The time points were at 1 min ligation-time (early effect) or at 15 min, 24 h or 48 h ligation-time (delayed application)

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For a 10mg vial, adding 1mL of bacteriostatic water creates a 10mg/mL concentration that works well across the full dose escalation range from 2mg (20 units) to 8mg (80 units)