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FCCP (10 M) was used 30 min prior to TMRE staining as a positive control of mitochondrial membrane potential loss

TEM evaluation further confirmed the existence of ferroptosis in airway epithelial cells in response to CS exposure and in COPD lungs

The samples were injected into an Agilent Eclipse Plus reversed-phase C18 column (2.1 mm 150 mm, 1.8 m particle size, 95 pore size) for separation

Under stress conditions, active HO-1 in the mitochondria regulates heme availability for mitochondrial cytochromes (Converso et al., 2006)

3f), suggesting that carbon flux to 2-OG is a rate-limiting step for glutamate production, which may be increased by the expression of CgCS and/or CitS