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Further attempts with new primers revealed a more detailed view onto the problem

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Following PBS-T washes (3 10 min), membranes were incubated with secondary antibodies (Licor IRDye 680RD Goat Anti Mouse- Cat#925-68070 and Licor IRDye 800CW Goat anti-Rabbit- Cat#926-32211), washed in PBS-T (3 10 min) and developed on a Licor Odyssey FC machine with western channel 700 and 800 and exposure time of 2 min

Cellular ROS levels in the TA were found to be lower than those in the EV and WT through a biochemical assay using the FOX reagent in the presence of H 2 O 2 (Figure 4B)