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Description

Came to this conclusion by looking at the chip block diagram as the source V and A comes from VDD

The supernatant was injected onto a 5-ml HisTrap HP column (Cytiva), and CENP-N/L complex was coeluted using 20 mM Tris, pH 7.5, 0.3 M NaCl, 5% glycerol, 5 mM BME, and 300 mM imidazole