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Preventing Microbial Contamination Always use sterile techniques and solvents

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[1] These effects are typically dose-dependent, most pronounced during dose escalation, and tend to diminish over time

Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose