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To test the role of these signaling systems, along with ATF4 during the acute activation of the ISR in hepatocytes, we evaluated short term changes in ten representative ISR genes after restriction of media methionine in an in vitro model of MR in HepG2 cells

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Importantly, one has to wait at least 4 weeks after the treatment was finished before re-testing is done to avoid getting false negative results

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After incubation, 50 L of K 3 [Fe(CN) 6 ] (2 mM) was added and the absorbance at 680 nm was recorded with a BioTek synergy 4 Multi-Mode Microplate Reader