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In research contexts, "stacking" refers to combination protocols that administer both compounds together or in sequence

Although, HSE has also been reported to be a trigger of brain autoimmunity by detecting anti-N-methyl-D-aspartate (anti-NMDAR) antibodies in some patients after HSE disease (Armangue et al., 2014), the potential relationship between HSV-1 and autoimmunity in MS patients has been largely discussed and remains controversial, as some studies have shown contradictory results (Koros et al., 2014

Cells were washed with ice-cold PBS, lysed in PBS containing 1% dodecyltrimethylammonium bromide in 0.2 M NaOH, and divided into two aliquotsone untreated and one treated with 1 M ascorbic acid followed by 0.4 M HCl

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