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The activation of IL-12p40 has previously been attributed to hypoxia-inducible factor-1, a key regulator of mucosal inflammation by controlling Th1/Th17 response [69]

Lastra G, Manrique CM, Hayden MR

Figure 2. A 25 kDa protein was expressed in E.coli and purified on an automated system using aone-step automated protocol with ProteIndex HiBond Ni-NTA Agarose 6 Fast Flow resin under native conditions. 50 mL E.coli lysate in 50 mM NaH2PO4, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL/min. The column was then washed with 100 mL 50 mM NaH2PO4, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL/min. The target protein was eluted with 50 mM NaH2PO4, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgents ProteIndex Ni-NTA 6FF (M) and Ni-Sepharose (G) resins

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The interface domain lies between the two polypeptide chains of the functional dimer of Sm TGR 28

Spatial cluster mapping and environmental modeling in pediatric inflammatory bowel disease