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For each child, age, gender, height, body weight, and liver function indicators, i.e., total protein (TP), albumin (ALB), alanine transaminase (ALT), aspartate transaminase (AST), glutamyltransferase (GGT), alkaline phosphatase (ALP), and total bilirubin (TBIL), were recorded

Isoquercitrin also promotes immunogenic cell death (ICD) in human gastric cancer cells through ER stress activation ( Sustained ER stress upregulates the C/EBP homologous protein (CHOP), a predominant pro-apoptotic transcription factor in ER, triggering downstream cascade responses (Kim et al., 2018)

Chrysin selectively downregulates MMP-10, demonstrating potent anti-metastatic effects in triple-negative breast cancer (TNBC) models (Monsef-Esfahani et al., 2014) Nepitrin, even at low concentrations, directly inhibits the enzymatic activity of MMP-2 and MMP-9, thereby attenuating proteolytic ECM remodeling (Yao et al., 2019), while luteolin suppresses MMP-2/9 expression to impair migration and invasion of cells of colorectal cancer and breast cancer (Li et al., 2015)

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To ensure comprehensive detection of pathogenic variants, a total of 21 long-range PCR primers were designed using NCBI Primer-BLAST to amplify key genomic regions of these genes [25]

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