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Immunocytochemical images were acquired with an automated microscope (PerkinElmer Operetta), quantitative image analysis was performed using Columbus software (PerkinElmer) and Harmony High-Content Imaging software (PerkinElmer)

Biol Trace Elem Res 2011

2023;109(1):e7687

Astragaloside-iv alleviates high glucose-induced ferroptosis in retinal pigment epithelial cells by disrupting the expression of mir-138-5p/sirt1/nrf2