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doi: 10.3390/ijms19041264 31 WoolardJBevanHSHarperSJBatesDO

Regulatory R region of the CFTR chloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions

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Materials and methods Generation of HEK293 cells stably expressing Grx1-roGFP1-iE ER HEK293 cells were transfected with Grx1-roGFP1-iE ER /pcDNA3.1 [13] using Metafectene PRO (Biontex) and stably expressing clones selected by addition of 1 mg/ml G418 (Sigma)

Cytoplasmic NADPH generation from the CPS relies upon cytoplasmic ACLY metabolizing CoA and mitochondrial-exported citrate for the generation of oxaloacetate and acetyl-CoA