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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

Stick to the recommended pea-sized amount to prevent irritation

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Instead, DIC mediates electro-neutral exchange of dicarboxylates or GSH for inorganic phosphate (Mari et al., 2009)

Unless we are malnourished, we arent truly deficient in this substance, but there are many benefits to increasing our intake of the nutrient