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To determine whether GSH depletion can also exert protective effects in a primarily MASH model, we overexpressed Chac1 in mouse liver using AAV8 followed by challenging mice with methionine/choline-deficient diets (MCDs) to induce MASH

For blood collection, the mice did not receive their alcohol bottles 1.5 h after drug administration on a 4-h session day, instead cheek blood was collected for biochemical analysis

Place the fibre pad on the clear panel (+ve) side, then place filter paper, membrane, gel ,filter paper, and fibre pad in the same order to make a sandwich

While 0 generally indicated no changes, the lesions were subsequently scored as follows: 0-3, edema (1- weak diffuse and/or perifocal