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Peak fractions with RNH1(D210N)-GFP-Tat11-His6, determined by SDS-PAGE, were pooled and diluted with buffer B [50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10% (v/v) glycerol, and 1 mM dithiothreitol (DTT)] to a final concentration of NaCl of 150 mM