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Immunoblotting Cell pellets were washed twice with ice-cold PBS before lysis in ice-cold RIPA buffer (10mM Tris-HCl, pH 7.5, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS) supplemented with protease inhibitors (Sigma-Aldrich, 11836170001) and phosphatase inhibitors (Roche, 04906837001)

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