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Imaging of assembloids and thin tissue sections was performed using an inverted multiphoton laser-scanning microscope (Zeiss LSM 780 NLO)

Gene-specific primers (Table S4) were used to amplify the target sequence with these cycles: first denaturation step for 2 min at 95 C, at least 15 cycles with 30 s at 95 C, annealing at 70 C for 1 min with a decreasing rate of 1 C/cycle until the melting temperature (T m

Moutsoglou, D

In the first few weeks, fine lines around the eyes, forehead, and mouth may become less visible, and your skin will look more radiant

PMID: 34725875 Cell ProlifSETD2 epidermal deficiency promotes cutaneous wound healing via activation of AKT/mTOR Signalling

This is further supported by studies (147), which identified deficits in nNOS neurons in various enteric neuropathies, including those associated with IBD