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Monitoring the SELEX procedure: melting curve and NGS analyses The melting temperature (Tm) profiles of amplified dsDNA (from eluted ssDNA) from different SELEX rounds were evaluated to decipher the diversity evolution of aptamer pools, as melting curve analysis is a rapid, simple, informative, and efficient approach for assessing the convergence of oligonucleotide pool diversity

Exosomal miR-146a contributes to the enhanced therapeutic efficacy of Interleukin-1-Primed mesenchymal stem cells against sepsis

In general, hydrophilic amino acids contribute to the formation of a hydrophobic pocket in the H-site adjacent to the GSH-binding site (Figure 3B&C) [80, 85]

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Conduct phase I/II clinical trials with rigorous pharmacokinetic/pharmacodynamic (PK/PD) monitoring, using validated biomarkers to ensure target engagement (e.g., reduced urinary 4-HNE levels) and safety (e.g., no iron deficiency)