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Description

Briefly, 1 mL of the extract sample was mixed with 0.5 mL FolinCiocalteus reagent (diluted 1:10 with dH 2 O), followed by the addition of 4 mL with sodium carbonate (75 gL 1 )

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Besides, we also confirmed that transferrin, another iron-binding protein, was increased post MI

Additionally, we should considerer that although both species and isolates from all phylogenetic lineages can infect humans and produce Paracoccidioidomycosis (PCM), they can also vary in virulence and induce different immune responses [68]