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J Exp Med (2018) 215(11):291935

One intriguing recent discovery is that gut microbes can produce small amounts of ammonia and other compounds that affect brain metabolism: a 2025 study showed that manipulating the gut microbiome altered brain amino acid levels and stress susceptibility in mice, partly via microbe-derived ammonia affecting neurotransmitter cycling [88]

Following incubation, a second centrifugation was carried out at 3000 g for 10 minutes to obtain the supernatant (whey), which was subsequently stored in 10 ml tubes at 20 C for further analysis

Thus, mitochondria are pivotal in regulating apoptosis mediated by iron and induced by cysteine deficiency (Gao et al., 2019) (Figure 1)

All uncropped and unedited blots are available in the Source Data