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HUVEC and HDMEC were maintained in Endothelial Cell Basal Medium (PromoCell) supplemented with 2% FCS, 0.4% endothelial cell growth supplement, 0.1 ng ml 1 recombinant human EGF (rh EGF), 1 ng ml 1 recombinant human basic fibroblast growth factor, 90 g ml 1 heparin and 1 g ml 1 hydrocortisone

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For pericyte coverage, FGF-2 amplifies the PDGF-B-PDGFR signaling at both ligand and receptor levels to ensure appropriate remodeling and maturation of tumor vessels (Fig

Following this dosing example, the vial will last about 7 injections

Zhou SL, Zhang XL, Wang JH

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