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Smad2 and Smad3 have differential sensitivity in relaying TGF signaling and inversely regulate early lineage specification

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Complete RPMI phenol red-free media (Gibco) was used and was beforehand completed with CellTox Green Dye at a 1:500 ratio (v/v)

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In contrast, N-terminus of Cagrilintide lies outside the transmembrane region, thereby avoiding interference with the binding pockets