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The expression of ARG1 in tumors has been observed to increase with tumor grade and reflects lymph node involvement, indicating a potential role in disease progression (153, 154)

The excess reagents were removed by methanol and chloroform (4:1) precipitation and samples were resolved on a 420% SDS-PAGE gel and for In-gel fluorescence, gels were imaged by Tanon 5200multi, for streptavidin blotting, after SDS-PAGE, the gels were transferred to a PVDF membrane, the blots were then blocked in 5% (w/v) milk in TBS-T (Tris-buffered saline, 0.1% Tween 20) for 1 hour at room temperature, the blots were stained with 0.3 g/mL HRP-labeled Streptavidin in TBS-T for 1 hour at room temperature and then washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Thermo Fisher) and imaging on the Tanon 5200multi

Murray, A

9 Enterome, Paris, France

however, it is unclear whether GABA can directly reduce the accumulation of toxic salt ions

Davies TF, Andersen S, Latif R, et al