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[46] [47] [48] Other analytical methods including mass spectrometry and NMR have also been applied, [18] and they are in particularly useful for the study of the coupling ratio between 2OG oxidation and substrate formation, and for the characterisation of unknown enzymatic products

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Finally, AcAc-CoA is split into two acetyl-CoAs by mitochondrial acetyl-CoA transferase (ACAT)