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Selecting neutral or zwitterionic lipids, controlling the encapsulation temperature (often below the protein's denaturation point), and adding stabilizing excipients such as trehalose or sucrose can significantly enhance protein preservation

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These include variability in synthesis protocols, inconsistent batch-to-batch reproducibility, and insufficient data on long-term toxicity and biodistribution

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Early evidence demonstrated that high levels of protein carbonyl groups, a general marker of OS, were present in both postmortem spinal cord [88, 89] and motor cortex [90] from sALS patients, compared to control samples