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When cells face oxidative stress, including metal-induced stress, KEAP1-mediated degradation of NFE2L2 is inhibited, leading to its stabilization and nuclear translocation

No significant deviation from the expected frequencies was detected ( P = 0.4744)

After removing the membrane and organelle fraction, the pellet was washed 3X with 100L MIB before resuspending the cytoskeletal and nuclear fraction in 125 L Cytoskeletal and Nuclear Isolation Buffer

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