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With the availability of the draft genome sequence for chickpeas, we have undertaken a re-analysis of the transcriptomes of chickpea root nodules and validated the newly identified expression patterns in two extremely contradictory nodulating genotypes namely ICC-1083 (lower) and ICC-9085 (higher) employing 8 treatments in triplicates through quantitative real-time PCR (qRT-PCR)

Nov 2019;11:100191

doi: 134

JabsWJTheissingENitschkeMBechtelJFMDuchrowMMohamedSet alLocal generation of C-reactive protein in diseased coronary artery venous bypass grafts and normal vascular tissue

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

In addition, it is essential to consider the destructive effect of elevated levels of vitamin C on erythrocytes and the potential occurrence of hemolysis (Shilotri and Bhat, 1977