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NF-B binding at the intronic enhancer region also enhances transcriptional activity of Sp1 in the proximal promoter of sod2 ( Posttranslational Regulation of Manganese Superoxide Dismutase Activity In addition to transcriptional regulation, previous reports indicated that Mn-SOD function is regulated by posttranslational modifications, including nitration, acetylation, phosphorylation, and glutathionylation

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Battula VL, Le PM, Sun JC, Sonnylal S, McQueen T, Ruvolo V, Michel KA, Ling X, Jacamo R, Shpall E, Wang Z, Kukkillaya ARU, Al-Altrash G, Konopleva M, Davis RE, Harrington MA, Palisch CW, Bueso-Ramos C, Andreeff M

Glutathione is a crucial antioxidant that helps protect the skin from an autoimmune response

Then, cell pellets were resuspended in 50 mM mannitol, 2 mM EGTA, 50 mM Tris-HCl, pH 7.0, supplemented with the protease inhibitor Cocktail (C0001, TargetMol, USA) and homogenized with a glass-Teflon tissue homogenizer

144 ReidM