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The labelled cells were subsequently washed again with warm PBS

After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1(GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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For example, some cancer cells have been shown to exhibit increased HMP shunt activity, which allows them to generate NADPH and biosynthetic intermediates required for rapid proliferation

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Depletion of intracellular GSH appears to be critical for subsequent alterations in protein thiol and calcium homeostasis