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Start by reducing MT2 dose by 3050% when introducing a second compound, especially if the new peptide has any overlapping mechanism or side effect profile

This lipotoxicity and oxidative stress vicious cycle creates a metabolic trap, which is particularly evident in glomerular mesangial cells and proximal tubular cells, thereby driving extracellular matrix deposition and the development of renal fibrosis

This stability, combined with tight mu-receptor binding, is why a seven-residue peptide can be several times more potent than morphine in animal assays [4]

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doi: 10.1016/j.bbrc.2016.11.017 135 GengJYangCWangBZhangXHuTGuYet al

(B) LDH release remains low across conditions, indicating no overt cytotoxicity during the assay