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This method relies on antibodies that recognize and bind to specific molecular structures

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As a cell surface receptor, CD38 is necessary for immune cell activation and proliferation

Prolotherapy Prolotherapy injects a neutral dextrose solution into knee tissues, initiating a local inflammatory response that triggers a healing cascade

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The sample was then centrifuged (Eppendorf 5810R, Hamburg, Germany) for 20 min (3220 g , RT) and the supernatant was collected and paper filtered (Whatman 597 1/2 ) for immunoaffinity purification