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Reconstituted solutions should generally not be used beyond 57 days even under refrigeration, and individual sequences with inherent instability (particularly those containing N, Q, C, M, or W residues) may require more conservative timelines

Regularly in prolonged regimen the last application was 24 hours before sacrifice

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This doesnt mean complete immobilization, which can impair tendon healing, but rather smart activity modification

The standard 4-6 weeks on, 2-4 weeks off pattern provides a structured approach even without specific data guiding these intervals

a distinct cuboidal shape, a high nucleus-to-cytoplasm ratio, sometimes binucleated, bright intercellular junctions and the presence of prominent lipid droplets in the cytoplasm