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As observed previously, the structure of CHT was normal in smarca5 zko1049a and the number of cmyb :GFP + HSPCs in CHT at 2 dpf was comparable between smarca5 zko1049a and their siblings (supplemental Figure 2A-B), indicating that the formation of blood clots in smarca5 zko1049a is dispensable for HSPC development in CHT