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Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer

Combined research protocols allow investigators to examine whether modulating NAD+ availability alongside mitochondrial redox activity produces measurable effects on cellular energy metabolism endpoints

Post-traumatic stress disorder may set the neurobiological stage for eating disorders: a focus on glutamatergic dysfunction

Cancer 3 , 113 (2022)

Diabetic and corticosteroid-impaired models are used to characterize these mechanisms under conditions of disrupted tissue repair