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Before incubation with the primary antibody, lipid droplets in bovine oocytes were cleared with 4,000 U/ml lipase from Candida rugose (Thermo Fisher Scientific) in 400 mM NaCl, 50 mM Tris (pH 7.2), 5 mM CaCl 2 , and 0.2% sodium taurocholate supplemented with ethylenediaminetetraacetic acid-free Protease Inhibitor Cocktail (11,836,170,001

Gibbs, 1999)

ablation of glial limitans prevents functional rehabilitation

Nutrient Dosing: In some applications, nutrients (such as nitrogen or phosphorus) are dosed to support microbial activity and prevent biofilm starvation

A gradual decline in the level of LPO was observed in this study following day 1 to day 30

Both oxidative damage to cells and reduced antioxidant capacity contribute to this disease