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Sample preparation Tissue sample Wash fresh tissues with PBS for twice, then add 0.1 g of animal/plant tissue into homogenizer (the homogenizer has been rinsed with Reagent I and placed on ice before use)

Hawasli A, Zonca S

Serotonin Serotonin regulates pleiotropic physiological processes, including cognition, circadian rhythm, nociception, blood coagulation, cardiovascular homeostasis, and gastrointestinal secretion and peristalsis (211)

This threshold provided us with the confidence to confidently attribute differential expression to specific genes within the unique context of our experiments