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Anyone on long-term metformin, PPIs, or a GLP-1 protocol with significantly reduced intake for months should have B12 checked as part of routine monitoring

So on the narrow question does it stop working after N weeks, the honest answer is: no published evidence says it does, and the mechanism actually argues against it

Toxicity induced by multiple high doses of vitamin B(12) during pernicious anemia treatment: a case report

GHK-Cu is compatible with most cell culture media but may interfere with BCA protein assays due to copper interference with the assay chemistry

In the absence of coenzyme B12, tetrahydrofolate cannot be regenerated from its inactive storage form, 5-methyltetrahydrofolate, and a functional folate deficiency occurs

Pricing Schedule: Month 1: $25 per week