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The secondary was then removed and the membrane was washed again in PBS-T, 3x for 15min

Medication (/kg) was given once daily intraperitoneally throughout the 7 days, with the last dose at 24 h before pressure assessment

The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well

doi: 10.1210/jcem.83.3.4674 162 OzerFFDagdelenSErbasT

Pinch approximately 2 to 5 cm of skin to lift the subcutaneous tissue away from underlying muscle

Because these synthetic peptides are manufactured cheaply using bacterial proteins, even a batch advertised at 99% purity contains a 1% remainder of bacterial protein toxicity