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Upon protonation at acidic pH, the positive surface charge of DC LNPs induces endosomal membrane fusion, thus facilitating the release of siRNA into the cytosol

10.1016/j.biopha.2019.108629 160 PasseronT.OrtonneJ

The background was subtracted for the sample measurements and each channel

Marengo B, Pulliero A, Izzotti A, Domenicotti C

Cryo-EM grid preparation and data collection For the cryo-EM grids preparation, 3 l of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV)

Use Misuse 53, 10681075