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Even with the right diet, the elderly can experience difficulty extracting the right nutrients from their foods, and this can lead to a vitamin deficiency

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Back Deacon, DF, Johnsen, AH, Holst, JJ (March 1995) 'Degradation of glucagon-like peptide-1 by human plasma in vitro yields an N-terminally truncated peptide that is a major endogenous metabolite in vivo', Journal Clinical Endocrinology Metabolism , 80/3, 952-7

Upon thawing, the solution may appear clear and normal, but the copper-binding activity is permanently destroyed