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Top-down MS alternative: ETD or EThcD fragmentation of the intact small fragment gives the N-terminal sequence in ~30 minutes if you have access to an Orbitrap with ETD

Lyophilized cakes may take fifteen to thirty minutes to fully hydrate

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[DOI] [PubMed] [Google Scholar] 62.Liu H., Peng L., Liu C., Tan Y., Xiang Y., Qu X., Weber H.C., Dai M., Qin X

Nevertheless, various stimuli such as chronic disease states, metabolic conditions (e.g., type 2 diabetes mellitus [T2DM], obesity, dyslipidemia), smoking, and disturbed blood flow can disrupt such a quiescent phenotype and lead to endothelial cell dysfunction (ECD) (Fig