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The membranes were blocked with 5% skim milk (BD Difco, NJ, United States) in PBS containing 0.05% Tween 20 buffer overnight at 4C and incubated with following primary antibodies for 2 h at room temperature: p38 MAPK antibody (dilution 1:1,000

Conclusion The present study demonstrates that both vitamin D supplementation and intermittent fasting exert significant hepatoprotective effects in a rat model of metabolic-associated steatotic liver disease

As mitochondrial metabolism generates free radicals, autophagic clearance of damaged mitochondria would limit the levels of oxidative and nitrosative damage (Giordano et al., 2014)

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Together, these mechanisms constitute a critical protective pathway against oxidative damage in multiple cell types [157]

When you reconstitute with bacteriostatic water (water containing 0.9% benzyl alcohol as preservative), you introduce water and initiate the degradation clock