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Entropic stabilization of the tryptophan synthase -subunit from a hyperthermophile, Pyrococcus furiosus : X-ray analysis and calorimetry

The mislocalization of Pf GS was induced by the addition of 250 nM rapamycin (Sigma-Aldrich, R0395) and verified by the translocation of GFP fluorescence from the parasite cytosol to the plasma membrane in live parasites

Bai Y, Cui W, Xin Y, et al

Therefore, for each pH both buffers were mixed in a 1:1 ratio and the pH of this solution was measured such that the final pH was known

Which translates to 0.028mL or roughly 3 units on an insulin syringe at 250mcg/0.1mL concentration

and Cao, J