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Together, these examples underscore the importance of studying metabolic remodeling during human neurodevelopment and emphasize the need for reliable, neuron-specific model systems that can recapitulate early stages of differentiation and bioenergetic specialization

Glutamate exocytosis from astrocytes controls synaptic strength

Statistical differences between groups were identified by one-way analysis of variance (ANOVA) followed by the Duncan post hoc test

After incubation at 4 C for 16 h, the streptavidin resin was collected and washed with 1% SDS (2X), 4 M urea (2X), PBS (2X) and ddH 2 O (2X), resuspended in loading buffer, boiled to release the biotin-conjugated proteins, and analyzed by immunoblot

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