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After TMB substrate solution is added, only those wells that contain Human MPIF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

The gels were imaged before and after and the extent of gel contraction measured using Image Pro Software

Following 3 h of drug treatments, 5 M of the cell-permeable, ROS-sensitive dye CellROX Deep Red (Invitrogen, Stockholm, Sweden) was added to each sample and incubated at 37 C for 30 min

Psychiatry 45 , 789796 (1988)

For our consistent 1mg dose example: (1mg dose / 2mg per mL) = 0.5 mL To find the units: 0.5 mL * 100 units/mL = 50 units

Journal of trace elements in medicine and biology : organ of the Societyfor Minerals and Trace Elements (GMS)