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Effects of topical creams containing vitamin C, a copper-binding peptide cream and melatonin compared with tretinoin on the ultrastructure of normal skina pilot clinical, histologic, and ultrastructural study

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FuGENE 6 Transfection Reagent (Promega) was used to transfect AC16 cells, which were seeded in 96-well plates (50,000 cells), with human PPARGC1A promoter containing pGL3-BV plasmids according to manufacturers protocols

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