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Eight non-structural proteins (Npro, p7, NS2, NS3, NS4A, NS4B, NS5A, NS5B) and four structural proteins (C, Erns, E1, E2) are produced by post-translational cleavage of the single ORF-encoded big polyprotein in ncp BVDV isolates 21

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For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

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