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Cells were incubated overnight at 4 C with anti-YAP D8H1X antibody (14074, cell Signaling, 1/100 dilution) in 1% BSA and 0.3% Triton X-100 followed by incubation for 1.5 h at room temperature with goat anti-rabbit Alexa fluor 594 (A-11012, Invitrogen, 1/500 dilution)

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By reacting statin drug precursors with oxalyl chloride, combining them with PHEMA (polyhydroxyethyl methacrylate), and coupling macrophage-targeting molecule ISO-1 and fluorescent polyethylene glycol via reagents such as dicyclohexylcarbodiimide, a multifunctional intelligent carrier was successfully constructed