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10) van Spreekens (1977) Biochemical changes induced by bacterial growth during storage and spoilage Comparison of the chemical compounds developing in naturally spoiling fish and sterile fish has shown that most of the volatile compounds are produced by bacteria (Shewan, 1962) as shown in Figure 5.11

It is also important to be aware of symptoms that may suggest either worsening B12 deficiency or poorly controlled blood glucose, and to seek prompt medical advice if you experience: Persistent fatigue, breathlessness, or pallor (possible anaemia) Tingling, numbness, or weakness in the hands or feet (possible B12-related neuropathy) Unsteadiness when walking, or changes in memory or thinking (possible signs of more severe B12 deficiency requiring prompt assessment) Increased thirst, frequent urination, or unexplained weight loss (possible hyperglycaemia) If you think you may be experiencing a side effect from a medicine for example, symptoms of B12 deficiency that you believe may be related to metformin you or your healthcare professional can report this via the MHRA Yellow Card scheme at yellowcard.mhra.gov.uk

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Recommendations may vary, but the minimum intake should be 400 mcg per day

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