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Ascorbate peroxidase in tea leaves: occurrence of two isozymes and differences in their enzymatic and molecular properties

After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 570 nm Precision pipettes to deliver 2 L to 1 mL volumes Tubes to prepare sample dilutions Incubator at 37C 50 mL conical tubes Deionized water Assay Procedure Summary Prepare the Reaction Mix (+LGOX), and Background Mix (-LGOX) before use

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Similarly, MYC binds directly to the promoter elements of glutamine transporters such as ASCT2 and SN2 in T cells to promote their mRNA synthesis, thereby increasing glutamine uptake and metabolism and supporting the activation and functional maintenance of immune cells [17]